Human CD163 Quantikine ELISA Kit Summary
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human CD163 in this assay. No medical histories were available for the donors used in this study.Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
Serum (n=36) | 472 | 88-902 | 186 |
Heparin plasma (n=36) | 521 | 186-996 | 203 |
Cell Culture Supernates:
Condition | Day 1 (ng/mL) | Day 6 (ng/mL) |
Unstimulated | ND | 5.0 |
Stimulated | ND | 4.4 |
Product Summary
Recovery
The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 107 | 101-110 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human CD163 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The sEVs miR-487a/Notch2/GATA3 axis promotes osteosarcoma lung metastasis by inducing macrophage polarization toward the M2-subtype
Authors: Wang, P;Yang, L;Dong, J;Liu, W;Xie, F;Lu, Y;Li, W;
Cancer cell international
Species: Human
Sample Types:
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Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells
Authors: Guan, X;Hu, R;Choi, Y;Srivats, S;Nabet, BY;Silva, J;McGinnis, L;Hendricks, R;Nutsch, K;Banta, KL;Duong, E;Dunkle, A;Chang, PS;Han, CJ;Mittman, S;Molden, N;Daggumati, P;Connolly, W;Johnson, M;Abreu, DR;Cho, BC;Italiano, A;Gil-Bazo, I;Felip, E;Mellman, I;Mariathasan, S;Shames, DS;Meng, R;Chiang, EY;Johnston, RJ;Patil, NS;
Nature
Species: Human
Sample Types: Serum
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HIV-associated gut microbial alterations are dependent on host and geographic context
Authors: Rocafort, M;Gootenberg, DB;Luévano, JM;Paer, JM;Hayward, MR;Bramante, JT;Ghebremichael, MS;Xu, J;Rogers, ZH;Munoz, AR;Okello, S;Kim, JH;Sentongo, R;Wagubi, R;Lankowski, A;Maruapula, S;Zhao, G;Handley, SA;Mosepele, M;Siedner, MJ;Kwon, DS;
Nature communications
Species: Human
Sample Types: Plasma
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Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity
Authors: Lischka, J;Schanzer, A;de Gier, C;Greber-Platzer, S;Zeyda, M;
Cytokine
Species: Human
Sample Types: Plasma
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Psychological stress is associated with arterial inflammation in people living with treated HIV infection
Authors: Chow, FC;Mundada, NS;Abohashem, S;La Joie, R;Iaccarino, L;Arechiga, VM;Swaminathan, S;Rabinovici, GD;Epel, ES;Tawakol, A;Hsue, PY;
Brain, behavior, and immunity
Species: Human
Sample Types: Plasma
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Soluble CD163 is a predictor of fibrosis and hepatocellular carcinoma development in nonalcoholic steatohepatitis
Authors: Kawanaka, M;Nishino, K;Kawada, M;Ishii, K;Tanikawa, T;Katsumata, R;Urata, N;Nakamura, J;Suehiro, M;Haruma, K;Kawamoto, H;
BMC gastroenterology
Species: Human
Sample Types: Serum
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Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia
Authors: Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert
Brain, Behavior, and Immunity, 2023-03-25;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Rebound HIV-1 in cerebrospinal fluid after antiviral therapy interruption is mainly clonally amplified R5 T cell-tropic virus
Authors: LP Kincer, SB Joseph, MM Gilleece, BM Hauser, S Sizemore, S Zhou, C Di Germani, H Zetterberg, D Fuchs, SG Deeks, S Spudich, M Gisslen, RW Price, R Swanstrom
Nature Microbiology, 2023-01-30;8(2):260-271.
Species: Human
Sample Types: CSF
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Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia
Authors: Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert
Brain, Behavior, and Immunity, 2023;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
Nature Microbiology, 2022-12-15;0(0):.
Species: Human
Sample Types: Serum
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Long-Term Associations between Human Cytomegalovirus Antibody Levels with All-Cause Mortality and Cardiovascular Outcomes in an Australian Community-Based Cohort
Authors: S Lee, N van den Be, A Castley, M Divitini, M Knuiman, P Price, D Nolan, F Sanfilippo, G Dwivedi
Viruses, 2022-11-29;14(12):.
Species: Human
Sample Types: Serum
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The biological activity of serum bacterial lipopolysaccharides associates with disease activity and likelihood of achieving remission in patients with rheumatoid arthritis
Authors: J Parantaine, G Barreto, R Koivuniemi, H Kautiainen, D Nordström, E Moilanen, M Hämäläinen, M Leirisalo-, K Nurmi, KK Eklund
Arthritis Research & Therapy, 2022-11-21;24(1):256.
Species: Human
Sample Types: Serum
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Longitudinal Assessment of Multiple Immunological and Inflammatory Parameters during Successful DAA Therapy in HCV Monoinfected and HIV/HCV Coinfected Subjects
Authors: P Zuccalà, T Latronico, R Marocco, S Savinelli, S Vita, F Mengoni, T Tieghi, C Borgo, B Kertusha, A Carraro, G D'Ettorre, V Vullo, CM Mastroiann, GM Liuzzi, M Lichtner
International Journal of Molecular Sciences, 2022-10-08;23(19):.
Species: Human
Sample Types: Plasma
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Heme oxygenase-1 as an important predictor of the severity of COVID-19
Authors: Y Hara, J Tsukiji, A Yabe, Y Onishi, H Hirose, M Yamamoto, M Kudo, T Kaneko, T Ebina
PLoS ONE, 2022-08-24;17(8):e0273500.
Species: Human
Sample Types: Serum
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Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration
Authors: JS Pedersen, MO Rygg, K Chrøis, EG Sustarsic, Z Gerhart-Hi, NJ Wever Albr, RR Serizawa, VB Kristianse, AL Basse, AEB Boilesen, BH Olsen, T Hansen, LL Gluud, S Madsbad, S Larsen, F Bendtsen, F Dela
Nature Communications, 2022-05-25;13(1):2931.
Species: Human
Sample Types: Plasma
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Inflammatory Markers after Switching to a Dual Drug Regimen in HIV-Infected Subjects: A Two-Year Follow-Up
Authors: M Vassallo, J Durant, R Fabre, L Lotte, A Sindt, A Puchois, A De Monte, R Cezar, P Corbeau, C Pradier
Viruses, 2022-04-28;14(5):.
Species: Human
Sample Types: Plasma
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Immune Activation in Primary Human Immunodeficiency Virus: Influence of Duration of Infection, Treatment, and Substance Use
Authors: T Gilada, SR Schnittman, E White, J Mercader, Y Wang, S Dasgupta, R Valdez, D Pinto-Sant, S Pasalar, J Sanchez, P Gonzales, JR Lama, R Bender Ign, A Duerr
Open forum infectious diseases, 2022-03-24;9(6):ofac155.
Species: Human
Sample Types: Plasma
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A matrix metalloproteinase-generated neoepitope of CRP can identify knee and multi-joint inflammation in osteoarthritis
Authors: LC Alexander, G McHorse, JL Huebner, AC Bay-Jensen, MA Karsdal, VB Kraus
Arthritis Research & Therapy, 2021-08-31;23(1):226.
Species: Human
Sample Types: Serum
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Assessing the differential impact of chronic CMV and treated HIV infection on CD8+ T-cell differentiation in a matched cohort study: is CMV the key?
Authors: MC Mueller, WV Kern, S Usadel, MC Pauly, T Cathomen, U Salzer
AIDS research and therapy, 2021-06-30;18(1):37.
Species: Human
Sample Types: Plasma
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Identification of distinct immune activation profiles in adult humans
Authors: R Cezar, A Winter, D Desigaud, M Pastore, L Kundura, AM Dupuy, C Cognot, T Vincent, C Reynes, C Dunyach-Re, JP Lavigne, R Sabatier, P Le Merre, E Maggia, P Corbeau
Scientific Reports, 2020-11-30;10(1):20824.
Species: Human
Sample Types: Plasma
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Impact of combined training with different exercise intensities on inflammatory and lipid markers in type 2 diabetes: a secondary analysis from a 1-year randomized controlled trial
Authors: JP Magalhães, DA Santos, IR Correia, M Hetheringt, R Ribeiro, JF Raposo, A Matos, MD Bicho, LB Sardinha
Cardiovasc Diabetol, 2020-10-07;19(1):169.
Species: Human
Sample Types: Plasma
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Interferon-alpha alters host glycosylation machinery during treated HIV infection
Authors: LB Giron, F Colomb, E Papasavvas, L Azzoni, X Yin, M Fair, A Anzurez, M Damra, K Mounzer, JR Kostman, P Tebas, U O'Doherty, H Tateno, Q Liu, MR Betts, LJ Montaner, M Abdel-Mohs
EBioMedicine, 2020-08-19;59(0):102945.
Species: Human
Sample Types: Plasma
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Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
Authors: LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs
Mucosal Immunol, 2020-03-09;0(0):.
Species: Human
Sample Types: Plasma
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Factor Xa Inhibition Reduces Coagulation Activity but Not Inflammation Among People With HIV: A Randomized Clinical Trial
Authors: JV Baker, J Wolfson, T Peterson, M Mooberry, M Gissel, H Mystakelis, MW Henderson, K Garcia-Mye, FS Rhame, TW Schacker, KE Brummel-Zi, I Sereti, NS Key, RP Tracy
Open Forum Infect Dis, 2020-02-01;7(2):ofaa026.
Species: Human
Sample Types: Serum
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Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses
Authors: Q Liu, Q Ou, H Chen, Y Gao, Y Liu, Y Xu, Q Ruan, W Zhang, L Shao
BMC Infect. Dis., 2019-11-28;19(1):1006.
Species: Human
Sample Types: Plasma
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Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey
Authors: G Guo, Y Zhu, Z Wu, H Ji, X Lu, Y Zhou, Y Li, X Cao, Y Lu, P Talbot, J Liao, Y Shi, H Bu
J. Cell. Mol. Med., 2018-07-11;0(0):.
Species: Primate
Sample Types: Serum
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Persistent high plasma levels of sCD163 and sCD14 in adult patients with measles virus infection
Authors: C Mascia, I Pozzetto, B Kertusha, R Marocco, C Del Borgo, T Tieghi, S Vita, S Savinelli, M Iannetta, V Vullo, M Lichtner, CM Mastroiann
PLoS ONE, 2018-05-24;13(5):e0198174.
Species: Human
Sample Types: Plasma
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Effect of influenza vaccine on tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in older adults
Authors: M Keshtkar-J, M Ouyang, M Keshtkarja, S Almed, H Li, JD Walston, R Rios, SX Leng
Vaccine, 2018-03-13;0(0):.
Species: Human
Sample Types: Serum
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Serum levels of soluble CD163 and CXCL5 may be predictive markers for immune-related adverse events in patients with advanced melanoma treated with nivolumab: a pilot study
Authors: T Fujimura, Y Sato, K Tanita, Y Kambayashi, A Otsuka, Y Fujisawa, K Yoshino, S Matsushita, T Funakoshi, H Hata, Y Yamamoto, H Uchi, Y Nonomura, R Tanaka, M Aoki, K Imafuku, H Okuhira, S Furudate, T Hidaka, S Aiba
Oncotarget, 2018-02-15;9(21):15542-15551.
Species: Human
Sample Types: Serum
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Reevaluation of immune activation in the era of cART and an aging HIV-infected population
Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
JCI Insight, 2017-10-19;2(20):.
Species: Human
Sample Types: Plasma
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Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
PLoS ONE, 2017-06-21;12(6):e0179400.
Species: Human
Sample Types: Plasma
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Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions
Authors: PA Alvarado-V, L Bernal, CA Paige, RL Grosick, C Moracho Vi, DW Ferreira, C Ulecia-Mor, EA Romero-San
Immunobiology, 2017-05-16;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV
Authors: SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke
Pathog Immun, 2017-05-02;2(1):138-150.
Species: Human
Sample Types: Plasma
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Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease
PLoS ONE, 2016-11-21;11(11):e0166954.
Species: Human
Sample Types: Plasma, Pleural Fluid
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Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
Open Forum Infect Dis, 2016-10-15;3(4):ofw224.
Species: Human
Sample Types: Plasma
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Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
PLoS ONE, 2015-12-04;10(12):e0144317.
Species: Human
Sample Types: Serum
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Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
PLoS ONE, 2014-12-29;9(12):e115226.
Species: Human
Sample Types: Plasma
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Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
PLoS ONE, 2014-08-18;9(8):e105442.
Species: Human
Sample Types: Plasma
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Reduced sTWEAK and increased sCD163 levels in HIV-infected patients: modulation by antiretroviral treatment, HIV replication and HCV co-infection.
Authors: Beltran L, Munoz Hernandez R, de Pablo Bernal R, Garcia Morillo J, Egido J, Noval M, Ferrando-Martinez S, Blanco-Colio L, Genebat M, Villar J, Moreno-Luna R, Moreno J
PLoS ONE, 2014-03-04;9(3):e90541.
Species: Human
Sample Types: Whole Blood
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Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system.
Authors: Forsvoll J, Kristoffersen E, Oymar K
Pediatr Rheumatol Online J, 2013-10-17;11(1):38.
Species: Human
Sample Types: Plasma
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CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
J Immunol, 2013-04-15;190(10):5267-78.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
Arthritis Res Ther, 2013-01-01;15(6):R212.
Species: Human
Sample Types: Plasma
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Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
Authors: Shimizu M, Yachie A
Cytokine, 2012-06-08;60(1):226-32.
Species: Human
Sample Types: Serum
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Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Authors: Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O
Arterioscler. Thromb. Vasc. Biol., 2010-03-18;30(6):1253-62.
Species: Human
Sample Types: Plasma
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Plasma was isolated from blood drawn in EDTA tubes from HIV+/ART and HIV- healthy controls. sCD163 was measured using the Quantikine elisa kit for human sCD163 (cat# DC1630) following the protocol supplied in the kit. Plasma samples were diluted 1:10 before use in the assay.
Figure legends:
a) Standard curve for sCD163 from which concentrations in plasma were extrapolated
b) Quantitated data from HIV+/ART and HIV- group.
Concentrations are expressed in ng/ml.