Human CD19 PerCP-conjugated Antibody

Catalog #: FAB4867C
2 Citations Datasheet / COA / SDS

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FAB4867C

Detection of CD19 in Human PBMCs by Flow Cytometry.

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All CD19 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD19 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD19.

Source

Monoclonal Mouse IgG₁ Clone # 4G7-2E3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CLL cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD19 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD19 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB4867C) or (B) Mouse IgG₁PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Alternate Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human CD19 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Frequency of regulatory B cells phenotypes in breast cancer patients in Egypt.
Authors: Elgendy S, El-Sabaa E, Ahmed S, Eid S, El-Feky M
Egypt J Immunol, 2021-01-01;28(1):12-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
J. Immunol., 2011-03-07;186(8):4967-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry