Human CD1b Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD1b

CD1b is a 44-46 kDa member of the CD1 family of proteins. It is one of five CD1 isoforms (CD1a-e) that resemble MHC-I type molecules. Based on structure, CD1a, 1b and 1c can be grouped together, with CD1d and 1e existing as stand-alone forms. CD1b is a 316 amino acid (aa) type I transmembrane glycoprotein that contains one Ig-like domain in its ECD. It is expressed in the late endosome/lysosome compartment of myeloid-type dendritic cells. Following synthesis, CD1b associates with SAP-C, binds two short lipids in an unusually large ligand-binding groove, and transits either to the plasma membrane, or more commonly, the endosomal compartment. In either case, exposure to Gm^- lipid A or mycobacterial glyolipid results in a substitution of the foreign lipid for the two short-chain self lipids, followed by its presentation on the plasma membrane. Select alpha beta and gamma δ T cell recognize these lipids and typically repond with Th1-type cytokines (IFN-gamma and TNF-alpha ). Unlike other CD1 family molecules, CD1b does not seem to have a soluble form. There is no rodent counterpart to human CD1b, only to CD1d.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• T Cell Antigen Recognition