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Human CD1b Antibody

Catalog #: MAB7446
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7446
MAB7446-SP
Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD1b Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD1b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm) CD1d1, or rmCD1d2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 737249
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1b
Ser18-Ser303
Accession # P29016
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD1b antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1b Monoclonal Antibody (Catalog # MAB7446, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD1b

CD1b is an approximately 40-50 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1b associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1b is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1b generates an additional isoform that lacks 55 amino acids of the Ig-like domain.

Entrez Gene IDs
910 (Human)
Alternate Names
CD1; CD1A; CD1b antigen; CD1B antigen, b polypeptide; CD1b molecule; CD1b; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; MGC125990; MGC125991; R1; T-cell surface glycoprotein CD1b

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Citation for Human CD1b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of changes in dendritic cell subsets that correlate with disease severity in dengue infection
    Authors: S Lertjuthap, L Khowawiset, R Keawvichit, K Polsrila, A Chuansumri, K Chokephaib, P Thitilertd, N Onlamoon, AA Ansari, K Pattanapan
    PLoS ONE, 2018-07-12;13(7):e0200564.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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