Human CD1b PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD1b

CD1b is a 44-46 kDa member of the CD1 family of proteins. It is one of five CD1 isoforms (CD1a-e) that resemble MHC-I type molecules. Based on structure, CD1a, 1b and 1c can be grouped together, with CD1d and 1e existing as stand-alone forms. CD1b is a 316 amino acid (aa) type I transmembrane glycoprotein that contains one Ig-like domain in its ECD. It is expressed in the late endosome/lysosome compartment of myeloid-type dendritic cells. Following synthesis, CD1b associates with SAP-C, binds two short lipids in an unusually large ligand-binding groove, and transits either to the plasma membrane, or more commonly, the endosomal compartment. In either case, exposure to Gm^- lipid A or mycobacterial glyolipid results in a substitution of the foreign lipid for the two short-chain self lipids, followed by its presentation on the plasma membrane. Select alpha beta and gamma δ T cell recognize these lipids and typically repond with Th1-type cytokines (IFN-gamma and TNF-alpha ). Unlike other CD1 family molecules, CD1b does not seem to have a soluble form. There is no rodent counterpart to human CD1b, only to CD1d.

Product Datasheets

Related Research Areas

• T Cell Antigen Recognition