Human CD1c/BDCA-1 PE-conjugated Antibody

Name: Human CD1c/BDCA-1 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB5910P

Catalog #: FAB5910P Datasheet / COA / SDS

Discontinued Product

FAB5910P has been discontinued.

View all CD1c/BDCA-1 products.

Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry.

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All CD1c/BDCA-1 Antibodies

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Human CD1c/BDCA-1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCA‑1
aa 19-302
Accession # P29017

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Goat Anti-Human CD1c/BDCA-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5910P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD1c/BDCA-1

CD1c (Cluster of differentiation antigen 1c) is a 43 kDa member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta ₂‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta ₂‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

Entrez Gene IDs

911 (Human)

Alternate Names

BDCA1; BDCA-1; c polypeptide; CD1; CD1A; CD1c antigen; CD1c molecule; CD1c; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; T-cell surface glycoprotein CD1c