Human CD229/SLAMF3 Antibody

Name: Human CD229/SLAMF3 Antibody
Brand: R&D Systems
SKU: MAB1898
Rating: 4 (1 reviews)

Catalog #: MAB1898
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1898-SP
MAB1898-500
MAB1898-100

All CD229/SLAMF3 Antibodies

Product Details

Citations (2)

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Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD229/SLAMF3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD229/SLAMF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse CD229 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 249936

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD229/SLAMF3
Lys48-Lys454
Accession # Q9HBG7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD229/SLAMF3 (Catalog # 1898-CD)

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood lymphocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD229/SLAMF3

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig‑like V-set and two Ig‑like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). The ECD of human CD229 shares 57%‑59% aa sequence identity with mouse and rat CD229. Within the first two Ig‑like domains that are common to all SLAM proteins, human CD229 shares 24%‑39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig‑like domain or short cytoplasmic region (2). CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells (2‑6). Homophilic binding between CD229 molecules is mediated by the N-terminal Ig‑like domain (7). Human and mouse CD229 exhibit cross-species binding (7). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell‑B cell contact (7). Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization (8, 9). In addition, there are two ITSMs which mediate phosphorylation-dependent CD229 association with SAP and SHP-2 (10). These four tyrosine-containing motifs are intact in the described CD229 splice variants. CD229 knockout mice show minimally impaired immune responses, suggesting functional redundancy with other molecules (11).

References

Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
de la Fuente, M.A. et al. (2001) Blood 97:3513.
Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
Romero, X. et al. (2004) Tissue Antigens 64:132.
Hogarth, P.M. et al. (1980) Immunogenetics 11:65.
Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
Romero, X. et al. (2005) J. Immunol. 174:7033.
Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
Martin, M. et al. (2005) J. Immunol. 174:5977.
Sayos, J. et al. (2001) Blood 97:3867.
Graham, D.B. et al. (2006) J. Immunol. 176:291.

Long Name

SLAM Family Member 3

Entrez Gene IDs

4063 (Human); 17085 (Mouse)

Alternate Names

CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD229/SLAMF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States
Authors: AJ Collier, SP Panula, JP Schell, P Chovanec, A Plaza Reye, S Petropoulo, AE Corcoran, R Walker, I Douagi, F Lanner, PJ Rugg-Gunn
Cell Stem Cell, 2017-03-23;0(0):.
Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
Haematologica, 2011-05-23;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot