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Human CD24 Antibody

Catalog #: AF5247
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5247
AF5247-SP
Detection of Human CD24 by Western Blot.
2 Images
Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD24 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD24 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD24
Ser27-Gly59
Accession # NP_037362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human colon tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD24 antibody by Western Blot. View Larger

Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry View Larger

Detection of CD24 in Human Colon. CD24 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) at 1 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24 (signal transducer 24; also HSA/heat shock antigen) is a variably glycosylated, 35‑50 kDa member of the CD24 family of proteins. It is a GPI‑linked sialoprotein that is expressed on B cell precursors and unactivated B cells, keratinocytes, and neutrophils and thymocytes. Ligation of CD24 appears to inhibit B cell differentiation, likely through the initiation of apoptosis. Potential CD24 ligands include P‑Selectin. The human CD24 precursor is 80 amino acids (aa) in length. It contains a 26 aa signal sequence plus a cleavable C‑terminal propeptide (aa 60‑80). There is one potential alternate start site at Met5. Over aa 27‑59, human CD24 shares only 30% aa identity with mouse CD24.

Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

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Citations for Human CD24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Calsequestrin 2 overexpression in breast cancer increases tumorigenesis and metastasis by modulating the tumor microenvironment
    Authors: Kim JH, Lee ES, Yun J et al.
    Molecular Oncology
  2. A pilot study on biological characteristics of human CD24(+) stem cells from the apical papilla
    Authors: J Liang, YJ Zhao, JQ Li, L Lan, WJ Tao, JY Wu
    Journal of dental sciences, 2021-04-24;17(1):264-275.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  3. CD24 regulates cancer stem cell (CSC)-like traits and a panel of CSC-related molecules serves as a non-invasive urinary biomarker for the detection of bladder cancer
    Authors: A Ooki, CJ VandenBuss, M Kates, NM Hahn, A Matoso, DJ McConkey, TJ Bivalacqua, MO Hoque
    Br. J. Cancer, 2018-10-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Arsenic Promotes the COX2/PGE2-SOX2 Axis to Increase the Malignant Stemness Properties of Urothelial Cells
    Authors: A Ooki, A Begum, L Marchionni, CJ VandenBuss, S Mao, K Max, MO Hoque
    Int. J. Cancer, 2018-02-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells
    Authors: W Cai, G Chen, Q Luo, J Liu, X Guo, T Zhang, F Ma, L Yuan, B Li, J Cai
    Mol. Cancer Ther, 2017-03-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Calsequestrin 2 overexpression in breast cancer increases tumorigenesis and metastasis by modulating the tumor microenvironment
    Authors: Kim JH, Lee ES, Yun J et al.
    Molecular Oncology

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