Human CD27 Ligand/TNFSF7 Antibody

Catalog #: MAB27381 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB27381-100
MAB27381-SP

Detection of CD27Ligand/TNFSF7 in THP-1 Human Cell Line by Flow Cytometry.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD27 Ligand/TNFSF7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD27 Ligand/TNFSF7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 832614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand
Gln45-Pro193
Accession # P32970

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD27Ligand/TNFSF7 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB27381, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Goat anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of CD27Ligand/TNFSF7 in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB27381) followed by Goat anti-mouse PE-conjugated secondary antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27 Ligand/TNFSF7

Human CD27 Ligand (also CD70 and TNFSF7) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.

Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Alternate Names

CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7