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Human CD3+ T Cell Enrichment Column, Small, Pack of 5

Catalog #: HTCC-5
10 Citations Datasheet / COA / SDS
Newer Version Available: MAGH101
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HTCC-5 has been discontinued and is replaced by MAGH101.

R&D Systems Cell Enrichment Column Kits: Antibody-coated Silica Beads
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Human CD3+ T Cell Enrichment Column, Small, Pack of 5 Summary

T Cell Enrichment Columns

T Cell Enrichment Columns Assay Principle
View Full Assay Principle

Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 37% and 54% of initial CD3+ cells loaded. The purity of recovered cells ranges from 87% to 95%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

  • Available for human, mouse, and rat cells
  • Purifies CD3+ T cells to 87 – 95%
  • Based upon gravitational flow
  • Simple procedure
  • No specialized equipment required

Kit Contents

  • Cell Selection Column
  • 10X Column Buffer

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

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Product Datasheet
COA

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Citations for Human CD3+ T Cell Enrichment Column, Small, Pack of 5

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Induction of tolerogenic dendritic cells by activated TGF-?/Akt/Smad2 signaling in RIG-I-deficient stemness-high human liver cancer cells
    Authors: M Zhong, C Zhong, W Cui, G Wang, G Zheng, L Li, J Zhang, R Ren, H Gao, T Wang, X Li, J Che, E Gohda
    BMC Cancer, 2019-05-14;19(1):439.  2019-05-14
  2. CD38+ M-MDSC expansion characterizes a subset of advanced colorectal cancer patients
    Authors: TA Karakashev, GA Dominguez, A Hashimoto, EW Lin, C Chiu, K Sasser, JW Lee, GL Beatty, DI Gabrilovic, AK Rustgi
    JCI Insight, 2018-03-22;3(6):.  2018-03-22
  3. Accumulation of myeloid-derived suppressor cells (MDSCs) induced by low levels of IL-6 correlates with poor prognosis in bladder cancer
    Authors: G Yang, W Shen, Y Zhang, M Liu, L Zhang, Q Liu, HH Lu, J Bo
    Oncotarget, 2017-06-13;8(24):38378-38388.  2017-06-13
  4. Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients
    Authors: T Condamine, GA Dominguez, JI Youn, AV Kossenkov, S Mony, K Alicea-Tor, E Tcyganov, A Hashimoto, Y Nefedova, C Lin, S Partlova, A Garfall, DT Vogl, X Xu, SC Knight, G Malietzis, GH Lee, E Eruslanov, SM Albelda, X Wang, JL Mehta, M Bewtra, A Rustgi, N Hockstein, R Witt, G Masters, B Nam, D Smirnov, MA Sepulveda, DI Gabrilovic
    Sci Immunol, 2016-08-05;1(2):.  2016-08-05
  5. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity.
    Authors: Lee-Chang, Catalina, Bodogai, Monica, Moritoh, Kanako, Olkhanud, Purevdor, Chan, Andrew C, Croft, Michael, Mattison, Julie A, Holst, Peter Jo, Gress, Ronald E, Ferrucci, Luigi, Hakim, Fran, Biragyn, Arya
    Blood, 2014-07-18;124(9):1450-9.  2014-07-18
  6. A novel role of hematopoietic CCL5 in promoting triple-negative mammary tumor progression by regulating generation of myeloid-derived suppressor cells.
    Authors: Zhang, Yan, Lv, Dandan, Kim, Ha-Jeong, Kurt, Robert A, Bu, Wen, Li, Yi, Ma, Xiaojing
    Cell Res, 2012-12-25;23(3):394-408.  2012-12-25
  7. Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.
    J Transl Med, 2012-05-16;10(0):95.  2012-05-16
  8. Identification of ghrelin receptor blocker, D-[Lys3] GHRP-6 as a CXCR4 receptor antagonist.
    Authors: Patel K, Dixit VD, Lee JH
    Int. J. Biol. Sci., 2011-11-26;8(1):108-17.  2011-11-26
  9. Implication of discoidin domain receptor 1 in T cell migration in three-dimensional collagen.
    Authors: Hachehouche LN, Chetoui N, Aoudjit F
    Mol. Immunol., 2010-03-29;47(9):1866-9.  2010-03-29
  10. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
    Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
    FASEB J, 2010-02-12;24(7):2558-66.  2010-02-12

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