Human CD300c Antibody

Catalog #: MAB3256 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3256-100
MAB3256-SP

Detection of CD300c in Human PBMC by Flow Cytometry.

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All CD300c Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD300c Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human CD300c in direct ELISA. In direct ELISAs, no cross-reactivity with Human CD300a, CD300e, CD300LG and Mouse CD300c was detected

Source

Recombinant Monoclonal Rabbit IgG Clone # 2799A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0-derived human CD300c protein
Met29-Arg183
Accession # Q08708

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.5 µg/10⁶ cells

Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD300c in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Rabbit Anti-Human CD300c Monoclonal Antibody (Catalog # MAB3256) or (B) Rabbit IgG Isotype Control Antibody (MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse anti-Human CD14 Phycoerythrin-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300c

Leukocyte mono-Ig-like receptor 2 (LMIR2; also CMRF-35, CMRF35-A antigen, and CD300c antigen) is a 23 kDa (predicted) type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of molecules within the immunoglobulin (Ig) superfamily (1-4). Human LMIR2 is synthesized as a 224 amino acid (aa) precursor that has a 20 aa signal sequence, a 163 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 20 aa cytoplasmic tail (SwissProt # Q08708). The ECD contains an Ig-like V-type domain (aa’s 22-128) and two N-linked glycosylation sites (aa’s 90 and 99). Downstream of the Ig V-domain, the membrane proximal region of LMIR2 (aa 128-183) contains a high proportion of proline (18%), serine (20%) and threonine (13%) residues (1). The transmembrane segment contains a charged glutamic acid that contributes to cell activation (1-3). Human LMIR2 shares 52% aa sequence identity with the mouse LMIR2, which is also known as CLM4. Human LMIR2 is 90% identical to human LMIR1 within the Ig-like domain. LMIR2 is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes (1, 3). Mouse LMIR2 has the characteristics of an activatory molecule capable of inducing cellular activation and effector function in most cells and macrophages (3). The ligand for LMIR2 is presently unknown.

References

Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
Clark, G.J. et al. (2001) Tissue Antigens 57:415.
Clark, G.J. et al. (2002) J. Biol. Regul. Homeost. Agents 16:233.
Daish, A. et al. (1993) Immunology 79:55.

Long Name

CD300 Antigen-Like Family Member C

Entrez Gene IDs

10871 (Human); 387565 (Mouse)

Alternate Names

CD300c antigenCMRF35 antigen; CD300c molecule; CD300c; CLM6; CLM-6; CMRF35 leukocyte immunoglobulin-like receptor; CMRF35; CMRF-35; CMRF35A leukocyte immunoglobulin-like receptor; CMRF-35A; CMRF35ACMRF35A1; CMRF35CMRF35-A1; CMRF35-like molecule 6; IGSF16 ; IgSF16; IGSF16CD300 antigen-like family member C; Immunoglobulin superfamily member 16; LIR