Human CD302/CLEC13A PE-conjugated Antibody

Name: Human CD302/CLEC13A PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB6367P

Catalog #: FAB6367P Datasheet / COA / SDS

Discontinued Product

FAB6367P has been discontinued.

View all CD302/CLEC13A products.

Detection of CD302/CLEC13A in Human Blood Monocytes by Flow Cytometry.

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All CD302/CLEC13A Antibodies

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Human CD302/CLEC13A PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD302/CLEC13A in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse CD302/CLEC13A is observed.

Source

Monoclonal Mouse IgG₁ Clone # 771910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD302/CLEC13A
Asp23-Asn167
Accession # Q81X05

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD302/CLEC13A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD302/CLEC13A PE-conjugated Monoclonal Antibody (Catalog # FAB6367P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD302/CLEC13A

CD302, also known as DCL-1 and CLEC13A, is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.

Entrez Gene IDs

9936 (Human); 66205 (Mouse); 295629 (Rat)

Alternate Names

BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1