Human CD36/SR-B3 Antibody Summary
Gly30-Asn439
Accession # P16671
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD36/SR‑B3 by Western Blot. Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD36/SR-B3 Mono-clonal Antibody (Catalog # MAB19552) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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CD36/SR‑B3 in Human Heart. CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19552) at 5 µg/mL for 1 hour at room temperature followed by incubation with Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human CD36/SR‑B3 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.5 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 138 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human CD36/SR‑B3 by Simple WesternTM. Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD36/SR-B3
CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta ‑amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.
- Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
- Khoury, J. et al. (2003) J. Exp. Med. 197:1657.
- Husemann, J. et al. (2002) Glia 40:195.
- Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
- Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Product Datasheets
Citation for Human CD36/SR-B3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Nanoparticle biointerfacing by platelet membrane cloaking.
Authors: Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen C, Kroll A, Carpenter C, Ramesh M, Qu V, Patel S, Zhu J, Shi W, Hofman F, Chen T, Gao W, Zhang K, Chien S, Zhang L
Nature, 2015-09-16;526(7571):118-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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