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Human CD36/SR-B3 Antibody

Catalog #: AF1955
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1955
AF1955-SP
Detection of Human CD36/SR‑B3 by Western Blot.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD36/SR-B3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD36 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CD36 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36
Gly30-Asn439
Accession # P16671
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10-50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD36/SR‑B3 antibody by Western Blot. View Larger

Detection of Human CD36/SR‑B3 by Western Blot. Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human CD36/SR-B3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD36/SR‑B3 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human CD36/SR-B3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD36/SR‑B3 by Simple WesternTM. Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR-B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1955) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References
  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
  3. Husemann, J. et al. (2002) Glia 40:195.
  4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
  5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Long Name
Scavenger Receptor Class B, Member 3
Entrez Gene IDs
948 (Human); 12491 (Mouse)
Alternate Names
BDPLT10; CD_antigen: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; CHDS7; cluster determinant 36; Collagen R; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIb; GPIV; PAS IV; PAS-4 protein; PAS-4; PASIV; Platelet collagen receptor; platelet glycoprotein 4; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

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Citations for Human CD36/SR-B3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Influence of maximal fat oxidation on long-term weight loss maintenance in humans
    Authors: S Dandanell, K Husted, S Amdisen, A Vigelsø, F Dela, S Larsen, JW Helge
    J. Appl. Physiol., 2017-05-25;0(0):jap.00270.201.
  2. Microvascular insulin resistance associates with enhanced muscle glucose disposal in CD36 deficiency
    Authors: Shibao, CA;Peche, VS;Williams, IM;Samovski, D;Pietka, TA;Abumrad, NN;Gamazon, E;Goldberg, I;Wasserman, D;Abumrad, NA;
    medRxiv : the preprint server for health sciences
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  3. Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36
    Authors: J Chean, CJ Chen, G Gugiu, P Wong, S Cha, H Li, T Nguyen, S Bhattichar, JE Shively
    The Journal of Biological Chemistry, 2021-10-16;0(0):101311.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  4. Circulating CD36 is increased in hyperlipidemic mice: Cellular sources and triggers of release
    Authors: Sudipta Biswas, Detao Gao, Jessica B. Altemus, Umar R. Rekhi, Ellen Chang, Maria Febbraio et al.
    Free Radical Biology and Medicine
  5. DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells
    Authors: A Suhail, ZA Rizvi, P Mujagond, SA Ali, P Gaur, M Singh, V Ahuja, A Awasthi, CV Srikanth
    Cell Rep, 2019-12-10;29(11):3522-3538.e7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training
    Authors: Ditte Søgaard, Marcin Baranowski, Steen Larsen, Michael Taulo Lund, Cathrine Munk Scheuer, Carina Vestergaard Abildskov et al.
    International Journal of Molecular Sciences
  7. Endothelial cell CD36 optimizes tissue fatty acid uptake
    Authors: Ni-Huiping Son, Debapriya Basu, Dmitri Samovski, Terri A. Pietka, Vivek S. Peche, Florian Willecke et al.
    Journal of Clinical Investigation
  8. Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis
    Authors: A Ladanyi, A Mukherjee, HA Kenny, A Johnson, AK Mitra, S Sundaresan, KM Nieman, G Pascual, SA Benitah, A Montag, SD Yamada, NA Abumrad, E Lengyel
    Oncogene, 2018-02-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation.
    Authors: Samovski D, Sun J, Pietka T, Gross R, Eckel R, Su X, Stahl P, Abumrad N
    Diabetes, 2014-08-25;64(2):353-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A novel ELISA for measuring CD36 protein in human adipose tissue.
    Authors: Allred CC, Krennmayr T, Koutsari C, Zhou L, Ali AH, Jensen MD
    J. Lipid Res., 2010-11-28;52(2):408-15.
    Species: Human
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: ELISA Development, Western Blot
  11. Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
    Authors: Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
    Circ. Res., 2009-07-30;105(5):500-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity.
    Authors: Mahadevan VS, Campbell M, McKeown PP, Bayraktutan U
    Cardiovasc. Res., 2006-06-27;72(1):60-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies
    Authors: WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa
    Cell Rep, 2019-12-10;29(11):3405-3420.e5.

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