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Human CD39L1/ENTPD2 Antibody

Catalog #: AF6087
1 Citation Datasheet / COA / SDS
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AF6087
AF6087-SP
Detection of Human CD39L1/ENTPD2 by Western Blot.
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Human CD39L1/ENTPD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD39L1 in direct ELISAs and Western blots.  In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse CD39L1 and less than 1% cross-reactivity with recombinant human (rh) CD39L2, rhCD39L3, and rhCD39L4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD39L1/ENTPD2
Thr29-Asp460
Accession # Q9Y5L3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human CD39L1/ENTPD2 (Catalog # 6087-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD39L1/ENTPD2 antibody by Western Blot. View Larger

Detection of Human CD39L1/ENTPD2 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6087) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L1/ENTPD2

CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca2+ and Mg2+ dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).

References
  1. Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on EctoATPases and Related Ectonucleotidases:18.
  2. Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
  3. Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.
  4. Sevigny, J. et al. 2002 Blood 99:2801.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 2
Entrez Gene IDs
954 (Human); 12496 (Mouse)
Alternate Names
CD39 antigen-like 1; CD39L1; CD39L1NTPDase 2; CD39-like-1; EC 3.6.1; EC 3.6.1.-; EC 3.6.1.5; Ecto-ATP diphosphohydrolase 2; Ecto-ATPase 2; Ecto-ATPase; Ecto-ATPDase 2; ectonucleoside triphosphate diphosphohydrolase 2; ENTPD2; NTPD2; NTPDase 2; NTPDase-2

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Citation for Human CD39L1/ENTPD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PSTi8 with metformin ameliorates perimenopause induced steatohepatitis associated ER stress by regulating SIRT-1/SREBP-1c axis
    Authors: P Singh, MI Reza, AA Syed, R Garg, A Husain, R Katekar, UK Goand, M Riyazuddin, AP Gupta, JR Gayen
    Heliyon, 2020-12-29;6(12):e05826.

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