Human CD39L4/ENTPD5 Antibody

Catalog #: AF5297 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5297
AF5297-SP

Detection of Human CD39L4/ENTPD5 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD39L4/ENTPD5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD39L4/ENTPD5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5
Val21-His428
Accession # O75356

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human CD39L4/ENTPD5 (Catalog # 5297-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD39L4/ENTPD5 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human CD39L4/ENTPD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L4/ENTPD5

CD39L4 (also ER-UDPase, PCPH and Ectonucleoside triphosphate diphosphohydrolase 5/eNTPDase5) is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation-dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa identity with mouse CD39L4.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 5

Entrez Gene IDs

957 (Human); 12499 (Mouse); 314312 (Rat)

Alternate Names

CD39 antigen-like 4; CD39L4; CD39L4NTPDase 5; CD39-like 4; EC 3.6.1; EC 3.6.1.42; EC 3.6.1.6; ectonucleoside triphosphate diphosphohydrolase 5; ENTPD5; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; MGC163357; MGC163359; NTPDase 5; NTPDase-5; Nucleoside diphosphatase; Pcph proto-oncogene protein; PCPH; proto-oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5