Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

Catalog #: FAB100MFV450 Datasheet / COA / SDS

Clone UCHT1 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB100MFV450-100

Detection of CD3 epsilon in PBMC lymphocytes by Flow Cytometry.

1 Image

All CD3 epsilon Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody Summary

Species Reactivity

Human

Specificity

Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex.

Source

Monoclonal Mouse IgG₁ Clone # UCHT1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human thymocytes (1)

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

mFluor Violet 450 SE (Excitation= 406 nm, Emission= 445 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD3 epsilon in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3 epsilon mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB100MFV450 or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2).

References

Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23:101.
McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York.
Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York.

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Alternate Names

CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE