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Human CD4 Antibody

Catalog #: MAB379
11 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB379-SP
MAB379-100
MAB379-500
CD4 in Human PBMCs.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 34930
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD4 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry/ Immunofluorescence Detection of Human CD4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human CD4 by Immunocytochemistry/Immunofluorescence CX3CR1 expressions in affected muscle of patients with polymyositis. The muscle tissues of polymyositis (PM) patients were double-stained with CD4, CD8, or CD68, as well as CX3CR1, and were analyzed with fluorescent microscopy: (A) CX3CR1, (B) CD4, (C) merged (A) and (B), (D) CX3CR1, (E) CD8, (F) merged (D) and (E), (G) CX3CR1, (H) CD68, and (I) merged (G) and (H). Arrows indicate double-positive cells. Original magnification, ×400. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar3761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human CD4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human CD4 by Immunocytochemistry/Immunofluorescence CX3CR1 expressions in affected muscle of patients with polymyositis. The muscle tissues of polymyositis (PM) patients were double-stained with CD4, CD8, or CD68, as well as CX3CR1, and were analyzed with fluorescent microscopy: (A) CX3CR1, (B) CD4, (C) merged (A) and (B), (D) CX3CR1, (E) CD8, (F) merged (D) and (E), (G) CX3CR1, (H) CD68, and (I) merged (G) and (H). Arrows indicate double-positive cells. Original magnification, ×400. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar3761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References
  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Human CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
    Authors: Komori M, Blake A, Greenwood M et al.
    Ann Neurol
  2. Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis
    Authors: Liping Qi, Kui Wu, Shutian Shi, Qingwei Ji, Huangtai Miao, Que Bin
    Experimental and Therapeutic Medicine
  3. Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp
    Authors: Gwanghui Ryu, Jun-Sang Bae, Ji Hye Kim, Eun Hee Kim, Lele Lyu, Young-Jun Chung et al.
    Allergy, Asthma & Immunology Research
  4. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
    Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
    J Neuroinflammation, 2017-12-15;14(1):248.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma
    Authors: P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang
    Oncol Lett, 2017-06-22;14(2):2458-2462.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Kv1.3 channels mark functionally competent CD8+ tumor infiltrating lymphocytes in head and neck cancer
    Cancer Res., 2016-11-04;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
    Authors: Suzuki F, Kubota T
    Arthritis Res. Ther., 2012-03-06;14(2):R48.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. The Scaffolding Protein Dlg1 Is a Negative Regulator of Cell-Free Virus Infectivity but Not of Cell-to-Cell HIV-1 Transmission in T Cells
    Authors: Patrycja Nzounza, Maxime Chazal, Chloé Guedj, Alain Schmitt, Jean-Marc Massé, Clotilde Randriamampita et al.
    PLoS ONE
  9. The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
    Authors: Schaecher SR, Diamond MS, Pekosz A
    J. Virol., 2008-07-16;82(19):9477-91.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  10. Presence of IgG-CD4 complexes in the circulation.
    Authors: Nezlin R, Bengtsson AA
    Immunol. Invest., 2008-01-01;37(2):153-62.
    Species: Human
    Sample Types: Serum
    Applications: Dot Blot
  11. Potentials and capabilities of the Extracellular Vesicle (EV) Array.
    Authors: Jorgensen MM, Baek R, Varming K.
    J Extracell Vesicles

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Reviews for Human CD4 Antibody

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Human CD4 Antibody
By Anonymous on 02/08/2021
Application: Octet binding assay Sample Tested: Recombinant protein Species: Human

Immobilize Anti-CD4, flow dose response of CD4 (200-3nM. KD ~5nM

Other Human CD4 Antibody MAB379
Human CD4 Antibody
By Anonymous on 01/22/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: activated mouse CD8 T cell Species: Human