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Human CD4 Biotinylated Antibody

Catalog #: BAF379
4 Citations Datasheet / COA / SDS
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BAF379
Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 (R&D Systems, Catalog # 514-CD)
Lys26-Trp390
Accession # P01730
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD4 (Catalog # 514-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References
  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Human CD4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
    Authors: Komori M, Blake A, Greenwood M et al.
    Ann Neurol
  2. Enhancement of Immune Effector Functions by Modulating IgG's Intrinsic Affinity for Target Antigen
    Authors: Yariv Mazor
    PLoS ONE, 2016-06-20;11(6):e0157788.
    Species: Human
    Sample Types: Buffer
    Applications: Bioassay
  3. Presence of IgG-CD4 complexes in the circulation.
    Authors: Nezlin R, Bengtsson AA
    Immunol. Invest., 2008-01-01;37(2):153-62.
    Species: Human
    Sample Types: Antibody
    Applications: Dot Blot
  4. Characterization of the antibody response elicited by HIV-1 Env glycomutants in rabbits.
    Authors: Reynard F, Willkomm N, Fatmi A
    Vaccine, 2006-08-07;25(3):535-46.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development

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