Human CD43 Antibody

Catalog #: AF2038
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2038
AF2038-SP

All CD43 Antibodies

Product Details

Citations (1)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD43 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD43 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD43
Ser20-Arg253
Accession # P16150

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD43

Flow Cytometry

0.25 µg/10⁶ cells

Human whole blood lymphocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD43

CD43 (leukosialin, sialophorin, also Ly-48 in mouse) is a type I transmembrane (TM) sialated mucin that is found on leukocytes and tumor cells. It is synthesized as a 397 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 123 aa cytoplasmic tail that contains an NLS. The extracellular region has 12% proline and 38% threonine and serine and shows both N- and O-linked glycosylation. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

Long Name

Cluster of Differentiation 43

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Alternate Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD43 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Specific and Novel microRNAs Are Regulated as Response to Fungal Infection in Human Dendritic Cells
Authors: A Dix, K Czakai, I Leonhardt, K Schäferhof, M Bonin, R Guthke, H Einsele, O Kurzai, J Löffler, J Linde
Front Microbiol, 2017-02-23;8(0):270.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot