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Human CD45 Biotinylated Antibody

Catalog #: BAM1430
12 Citations Datasheet / COA / SDS
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BAM1430
CD45 in human PBMCs.
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Citations (12)
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Human CD45 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD45. This antibody recognizes all isoforms of human CD45.
Source
Monoclonal Mouse IgG1 Clone # 2D1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human peripheral blood mononuclear cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD45 antibody in human PBMCs by Immunocytochemistry (ICC). View Larger

CD45 in human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD45 Biotinylated Monoclonal Antibody (Catalog # BAM1430) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily
O-glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Human CD45 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. DNA hypomethylation silences anti-tumor immune genes in early prostate cancer and CTCs
    Authors: Hongshan Guo, Joanna A. Vuille, Ben S. Wittner, Emily M. Lachtara, Yu Hou, Maoxuan Lin et al.
    Cell
  2. HIF1A signaling selectively supports proliferation of breast cancer in the brain
    Authors: RY Ebright, MA Zachariah, DS Micalizzi, BS Wittner, KL Niederhoff, LT Nieman, B Chirn, DF Wiley, B Wesley, B Shaw, E Nieblas-Be, L Atlas, A Szabolcs, AJ Iafrate, M Toner, DT Ting, PK Brastianos, DA Haber, S Maheswaran
    Nature Communications, 2020-12-09;11(1):6311.
    Species: Human
    Sample Types: Whole Blood
    Applications: Neutralization
  3. Pancreatic circulating tumor cell profiling identifies LIN28B as a metastasis driver and drug target
    Authors: JW Franses, J Philipp, P Missios, I Bhan, A Liu, C Yashaswini, E Tai, H Zhu, M Ligorio, B Nicholson, EM Tassoni, N Desai, AS Kulkarni, A Szabolcs, TS Hong, AS Liss, C Fernandez-, DP Ryan, S Maheswaran, DA Haber, GQ Daley, DT Ting
    Nat Commun, 2020-07-03;11(1):3303.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. AR Expression in Breast Cancer CTCs Associates with Bone Metastases
    Authors: Nicola Aceto, Aditya Bardia, Ben S. Wittner, Maria C. Donaldson, Ryan O'Keefe, Amanda Engstrom et al.
    Molecular Cancer Research
  5. Molecular signatures of circulating melanoma cells for monitoring early response to immune checkpoint therapy
    Authors: X Hong, RJ Sullivan, M Kalinich, TT Kwan, A Giobbie-Hu, S Pan, JA LiCausi, JD Milner, LT Nieman, BS Wittner, U Ho, T Chen, R Kapur, DP Lawrence, KT Flaherty, LV Sequist, S Ramaswamy, DT Miyamoto, M Lawrence, M Toner, KJ Isselbache, S Maheswaran, DA Haber
    Proc. Natl. Acad. Sci. U.S.A., 2018-02-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  6. Improved Detection of Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasms
    Authors: Joseph W. Franses, Omer Basar, Abdurrahman Kadayifci, Osman Yuksel, Melissa Choz, Anupriya S. Kulkarni et al.
    The Oncologist
  7. Monitoring Neutropenia for Cancer Patients at the Point of Care
    Authors: Hakan Inan, James L. Kingsley, Mehmet O. Ozen, Huseyin Cumhur Tekin, Christian R. Hoerner, Yoriko Imae et al.
    Small Methods
  8. Expression of ?-globin by cancer cells promotes cell survival during blood-borne dissemination
    Authors: Y Zheng, DT Miyamoto, BS Wittner, JP Sullivan, N Aceto, NV Jordan, M Yu, NM Karabacak, V Comaills, R Morris, R Desai, N Desai, E Emmons, JD Milner, RJ Lee, CL Wu, LV Sequist, W Haas, DT Ting, M Toner, S Ramaswamy, S Maheswaran, DA Haber
    Nat Commun, 2017-02-09;8(0):14344.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  9. HER2 expression identifies dynamic functional states within circulating breast cancer cells
    Nature, 2016-08-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
    Authors: Tseng C, Biancotti J, Berg B, Gate D, Kolar S, Muller S, Rodriguez M, Rezai-Zadeh K, Fan X, Beenhouwer D, Town T, Liu G
    PLoS Pathog, 2015-11-30;11(11):e1005292.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Single-Cell RNA Sequencing Identifies Extracellular Matrix Gene Expression by Pancreatic Circulating Tumor Cells
    Authors: David T. Ting, Ben S. Wittner, Matteo Ligorio, Nicole Vincent Vincent Jordan, Ajay M. Shah, David T. Miyamoto et al.
    Cell Reports
  12. Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes.
    Authors: Brownell J, Wagoner J, Lovelace E, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Crispe I, Rosen H, Polyak S
    J Hepatol, 2013-06-12;59(4):701-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection

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