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Human CD46 Antibody

Catalog #: MAB2005
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2005
MAB2005-SP
Detection of CD46 in PBMC lymphocytes by Flow Cytometry
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD46 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD46 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 344519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD46
Cys35-Asp328
Accession # NP_722548
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD46
Flow Cytometry
2.5 µg/106 cells
see below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

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Detection of CD46 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD46 Monoclonal Antibody (Catalog # MAB2005, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD46

CD46, also known as MCP, is a type I membrane protein that functions as a cofactor for Complement Factor I-mediated inactivation of complement components C3b and C4b. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. The extracellular region of CD46 contains four tandem Sushi domains. At least fourteen transcript variants encode isoforms with deletions or substitutions following the fourth Sushi domain. Within corresponding regions, human CD46 shares 50% amino acid sequence identity with mouse and rat CD46.

Entrez Gene IDs
4179 (Human); 17221 (Mouse)
Alternate Names
AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen

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Citations for Human CD46 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus
    Authors: U Ellinghaus, A Cortini, CL Pinder, GL Friec, C Kemper, TJ Vyse
    Eur. J. Immunol., 2017-05-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. A novel factor I activity in Nipah virus inhibits human complement pathways through cleavage of C3b.
    Authors: Johnson J, Borisevich V, Rockx B, Parks G
    J Virol, 2014-10-29;89(2):989-98.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  3. The paramyxoviruses simian virus 5 and mumps virus recruit host cell CD46 to evade complement-mediated neutralization.
    Authors: Johnson JB, Grant K, Parks GD
    J. Virol., 2009-05-20;83(15):7602-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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