Human CD53 APC-conjugated Antibody

Catalog #: FAB4624A Datasheet / COA / SDS

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FAB4624A

Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

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Human CD53 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 425514

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 APC-conjugated Monoclonal Antibody (Catalog # FAB4624A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein that belongs to the 4 TM tetraspanin superfamily of molecules. CD53 is widely expressed on hematopoietic cells, including eosinophils, neutrophils, mast cells, CD16⁺ proinflammatory monocytes, NK cells T cells, macrophages, splenic B cells and immature dendritic cells. Ligation of CD53 promotes cell proliferation and survival, as well as homotypic cell‑cell adhesion. On the cell membrane, CD53 associates with other tetraspanins, MHC class II molecules, and alpha 3 and alpha 4 integrins. Human CD53 shares 71% aa sequence identity with mouse and CD53 in the extracellular domains.

Entrez Gene IDs

963 (Human); 12508 (Mouse); 24251 (Rat)

Alternate Names

antigen MOX44 identified by monoclonal MRC-OX44; CD53 antigentetraspanin-25; CD53 glycoprotein; CD53 molecule; CD53 tetraspan antigen; CD53; cell surface antigen; Cell surface glycoprotein CD53; MOX44; MOX44transmembrane glycoprotein; Tetraspanin-25; TSPAN25; tspan-25; TSPAN25leukocyte surface antigen CD53