Human CD68/SR-D1 PE-conjugated Antibody Summary
Asn22-Ile320
Accession # AAB25811
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD68/SR‑D1 in Human Macrophages by Flow Cytometry. Human CD14+ monocytes were selected from PBMCs using MagCellect Human CD14+ Cell Isolation Kit (MAGH105) and cultured for four days in recombinant human M-CSF (216-MC; 10 ng/mL) then stained with (A) Mouse Anti-Human CD68/SR-D1 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # IC20401P) or (B) isotype control antibody (IC0041P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of CD68/SR‑D1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD68/SR-D1 PE-conjugated Monoclonal Antibody (Catalog # IC20401P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD68/SR-D1
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.
Product Datasheets
Citations for Human CD68/SR-D1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Inhibition of osteoclastogenesis by opsonized Porphyromonas gingivalis
Authors: Subramanya N. Pandruvada, Jeffrey L. Ebersole, Sarandeep S. Huja
FASEB BioAdvances
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Statins Modulate Microenvironmental Cues Driving Macrophage Polarization in Simulated Periodontal Inflammation
Authors: Waleed Alkakhan, Nico Farrar, Vanessa Sikora, Pinar Emecen-Huja, Sarandeep S. Huja, Özlem Yilmaz et al.
Cells
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Complement inhibitor factor H expressed by breast cancer cells differentiates CD14+ human monocytes into immunosuppressive macrophages
Authors: Karolina I. Smolag, Christine M. Mueni, Karin Leandersson, Karin Jirström, Catharina Hagerling, Matthias Mörgelin et al.
OncoImmunology
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Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesisviarecruitment of different macrophage subtypes
Authors: L Chen, GQ Liu, HY Wu, J Jin, X Yin, D Li, PR Lu
Int J Ophthalmol, 2018-02-18;11(2):216-222.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
Am. J. Pathol., 2010-09-09;177(5):2472-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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