Human CD79A Antibody

Catalog #: MAB6920 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6920
MAB6920-SP

Detection of Human CD79A by Western Blot.

2 Images

All CD79A Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD79A Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD79A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD79A is observed.

Source

Monoclonal Mouse IgG_2B Clone # 706962

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD79A
Leu33-Arg143
Accession # P11912

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Lymph Node

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human CD79A by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB6920) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for CD79A at approximately 44 to 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

Detection of CD79A in Human Lymph Node. CD79A was detected in immersion fixed paraffin-embedded sections of Human Lymph Node using Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB6920) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD79A

CD79A, also known as Ig alpha and MB-1, is a 44 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39 aa deletion in the ECD. Heterodimers of CD79A and CD79B/Ig beta associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% aa sequence identity with mouse and rat CD79A.

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat); 722190 (Rhesus Macaque)

Alternate Names

CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein