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Human CD83 PE-conjugated Antibody

Catalog #: FAB1774P
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1774P
Detection of CD83 in CD14+ Monocyte-derived Dendritic Cells by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD83 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD83 in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # HB15e
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
CD14+ monocyte-derived dendritic cells and Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD83 antibody in CD14+ Monocyte-derived Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of CD83 in CD14+ Monocyte-derived Dendritic Cells by Flow Cytometry. CD14+ monocyte-derived dendritic cells were stained with Mouse Anti-Human CD83 PE-conjugated Monoclonal Antibody (Catalog # FAB1774P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CD83 in Daudi cells by Flow Cytometry. Daudi cells were stained with Mouse Anti-Human CD83 PE‑conjugated Monoclonal Antibody (Catalog # FAB1774P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD83

Human CD83 is a 40‑50 kDa member of the Siglec (or Sialic-acid-binding Immunoglobulin-like Lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

References
  1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
  2. Kozlow, E.J. et al. (1993) Blood 81:454.
  3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
  9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  10. Yamashiro, S. et al. (2000) Blood 96:3958.
  11. Cao, W. et al. (2005) Biochem. J. 385:85.
  12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
  13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  14. Hirano, N. et al. (2006) Blood 107:1528.
  15. Kotzor, N. et al. (2004) Immunobiology 209:129.
  16. Zinser, E. et al. (2006) Immunobiology 211:449.
  17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
Entrez Gene IDs
9308 (Human); 12522 (Mouse)
Alternate Names
B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83

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Citations for Human CD83 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell‑dendritic cell‑based vaccine
    Authors: Mona S Abdellateif, Sabry M Shaarawy, Eman Z Kandeel, Ahmed H El‑Habashy, Mohamed L Salem, Motawa E El‑Houseini
    Oncology Letters
  2. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020-04-21;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
    Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
    Clin. Cancer Res., 2008-08-01;14(15):4898-907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Eosinophils induce DC maturation, regulating immunity.
    Authors: Lotfi R, Lotze MT
    J. Leukoc. Biol., 2007-11-08;83(3):456-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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