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Human CD94 Fluorescein-conjugated Antibody

Catalog #: FAB1058F
2 Citations Datasheet / COA / SDS

Discontinued Product

FAB1058F has been discontinued.
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Detection of CD94 in Human PBMCs by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD94 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.
Source
Monoclonal Mouse IgG1 Clone # 131412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD94 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD94 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human CD94 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1058F) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD94

CD94 is a 30 kDa type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer (NK) cells and a subset of CD8+ T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with a NKG2 family member. The CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, the CD94/NKG2C heterodimer associates with the DAP12 adaptor protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins.

Entrez Gene IDs
3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor

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Citations for Human CD94 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Plasmodium falciparum-infected erythrocytes induce granzyme B by NK cells through expression of host-Hsp70.
    Authors: Bottger E, Multhoff G, Kun JF, Esen M
    PLoS ONE, 2012-03-15;7(3):e33774.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH 17 cytokine interleukin-22.
    Authors: Hughes T, Becknell B, McClory S, Briercheck E, Freud AG, Zhang X, Mao H, Nuovo G, Yu J, Caligiuri MA
    Blood, 2009-02-24;113(17):4008-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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