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Human CDCP1 Antibody

Catalog #: AF2666
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2666
AF2666-SP
CDCP1 in Human Colon Cancer Tissue.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CDCP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDCP1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Phe30-Leu666
Accession # NP_073753
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CDCP1
Flow Cytometry
2.5 µg/106 cells
SW480 human colorectal adenocarcinoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CDCP1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CDCP1 in Human Colon Cancer Tissue. CDCP1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Long Name
CUB Domain Containing Protein 1
Entrez Gene IDs
64866 (Human); 109332 (Mouse)
Alternate Names
CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

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Citations for Human CDCP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Effective targeting of intact and proteolysed CDCP1 for imaging and treatment of pancreatic ductal adenocarcinoma
    Authors: T Kryza, T Khan, S Puttick, C Li, KA Sokolowski, BW Tse, T Cuda, N Lyons, M Gough, J Yin, A Parkin, EI Deryugina, JP Quigley, RHP Law, JC Whisstock, AD Riddell, AP Barbour, DK Wyld, PA Thomas, S Rose, CE Snell, M Pajic, Y He, JD Hooper
    Theranostics, 2020-03-04;10(9):4116-4133.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Development of an enzyme-linked immunosorbent assay for detection of CDCP1 shed from the cell surface and present in colorectal cancer serum specimens
    Authors: Yang Chen, Brittney S. Harrington, Kevin C.N. Lau, Lez J. Burke, Yaowu He, Mary Iconomou et al.
    Journal of Pharmaceutical and Biomedical Analysis
  3. Dysregulated expression of cell surface glycoprotein CDCP1 in prostate cancer
    Authors: Lifang Yang, Sucharita M. Dutta, Dean A. Troyer, Jefferson B. Lin, Raymond A. Lance, Julius O. Nyalwidhe et al.
    Oncotarget
  4. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Authors: Kelly OG, Chan MY, Martinson LA
    Nat. Biotechnol., 2011-07-31;29(8):750-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Proteolysis-induced N-terminal ectodomain shedding of the integral membrane glycoprotein CUB domain-containing protein 1 (CDCP1) is accompanied by tyrosine phosphorylation of its C-terminal domain and recruitment of Src and PKCdelta.
    Authors: He Y, Wortmann A, Burke LJ
    J. Biol. Chem., 2010-06-15;285(34):26162-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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