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Human CEACAM-1/CD66a PE-conjugated Antibody

Catalog #: FAB2244P
10 Citations 1 Review Datasheet / COA / SDS
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FAB2244P
Detection of CEACAM‑1/CD66a in Human Blood Granulocytes by Flow Cytometry.
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Citations (10)
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Human CEACAM-1/CD66a PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1.
Source
Monoclonal Mouse IgG2B Clone # 283340
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM-1
Gln35-Gly428
Accession # P13688
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM‑1/CD66a antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CEACAM‑1/CD66a in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Fc gamma RIII (CD16) CFS-conjugated Monoclonal Antibody (Catalog # FAB2546F) and either (A) Mouse Anti-Human CEACAM-1/CD66a PE-conjugated Monoclonal Antibody (Catalog # FAB2244P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-1/CD66a

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V‑type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V‑type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Thompson, J. et al. (1992) Genomics 12:761.
  3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
  4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
  5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
  6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
  7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
  8. Watt, S.M. et al. (1994) Blood 84:200.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
  11. Bogoevska, V. et al. (2005) Glycobiology 16:197.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Entrez Gene IDs
634 (Human); 26365 (Mouse); 102139857 (Cynomolgus Monkey)
Alternate Names
antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR

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Citations for Human CEACAM-1/CD66a PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency
    Authors: Beliakova-Bethell N, Manousopoulou A, Deshmukh S et al.
    Virology
  2. CEACAM1 is a novel culture-compatible surface marker of expanded long-term reconstituting hematopoietic stem cells
    Authors: Unain Ansari, Elisa Tomellini, Jalila Chagraoui, Bernhard Lehnertz, Nadine Mayotte, Marie-Eve Bordeleau et al.
    Blood Advances
  3. Proinflammatory and Cancer-Promoting Pathobiont Fusobacterium nucleatum Directly Targets Colorectal Cancer Stem Cells
    Authors: V Cavallucci, I Palucci, M Fidaleo, A Mercuri, L Masi, V Emoli, G Bianchetti, ME Fiori, G Bachrach, F Scaldaferr, G Maulucci, G Delogu, G Pani
    Biomolecules, 2022-09-07;12(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interferon regulatory factor 1 and a variant of heterogeneous nuclear ribonucleoprotein L coordinately silence the gene for adhesion protein CEACAM1
    Authors: KJ Dery, C Silver, L Yang, JE Shively
    J. Biol. Chem., 2018-05-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Assessing the Impact of Targeting CEACAM1 in Head and Neck Squamous Cell Carcinoma
    Authors: K Tam, DW Schoppy, JH Shin, JK Tay, U Moreno-Nie, DC Mundy, JB Sunwoo
    Otolaryngol Head Neck Surg, 2018-02-13;0(0):1945998187566.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis
    Authors: F Gebauer, D Wicklein, M Tachezy, T Grob, D Steinemann, G Manukjan, G Göhring, B Schlegelbe, H Maar, JR Izbicki, M Bockhorn, U Schumacher
    Anticancer Res, 2016-04-01;36(4):1507-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Moraxella catarrhalis adhesin UspA1-derived recombinant fragment rD-7 induces monocyte differentiation to CD14+CD206+ phenotype.
    Authors: Xie, Qi, Brackenbury, Louise S, Hill, Darryl J, Williams, Neil A, Qu, Xun, Virji, Mumtaz
    PLoS ONE, 2014-03-05;9(3):e90999.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014-01-29;9(1):e84417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CEACAM1 regulates Fas-mediated apoptosis in Jurkat T-cells via its interaction with beta-catenin.
    Authors: Li Y, Shively J
    Exp Cell Res, 2013-03-07;319(8):1061-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CEACAM 1, 3, 5 and 6 -positive classical monocytes correlate with interstitial lung disease in early systemic sclerosis
    Authors: Kana Yokoyama, Hiroki Mitoma, Shotaro Kawano, Yusuke Yamauchi, Qiaolei Wang, Masahiro Ayano et al.
    Frontiers in Immunology

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Human CEACAM-1/CD66a PE-conjugated Antibody
By Vincent Giacalone on 07/27/2020
Application: Flow Sample Tested: Peripheral blood neutrophils Species: Human

This antibody was used to stain human peripheral whole blood for analysis by flow cytometry. Neutrophils were identified from total live CD45+ leukocytes and expression of CD66a was measured in the PE channel. Consistent fluorescence is observed after fixation in 4% PFA. Good antibody for staining granulocytes

Flow Cytometry Human CEACAM-1/CD66a PE-conjugated Antibody FAB2244P