Human CEBP  alpha Antibody

CEBP alpha in Human Liver. CEBP a was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CEBP a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7094) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.