Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CEBP alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEBP  alpha
Met1-Ala124
Accession # P49715

Specificity

Detects human CEBP  alpha in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CEBP beta, rhCEBP gamma, rhCEBP delta, rhCEBP zeta, and rhCEBP epsilon is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CEBP  alpha Antibody

CEBP a antibody in Human Liver by Immunohistochemistry (IHC-P).

CEBP alpha in Human Liver.

CEBP a was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CEBP a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7094) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Detection of Human CEBP alpha by Immunocytochemistry/Immunofluorescence

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CEBP  alpha Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEBP alpha

CEBP alpha (CCAAT/Enhancer-Binding Protein; also C/EBP) is a 42-43 kDa member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP alpha  is 358 amino acids (aa) in length). It contains one transactivation domain (aa 1-96), poly-Gly segment (aa 99-104), a poly-Pro motif (aa 183-189), a bZIP dimerization region (aa 281-334), and an overlapping DNA-binding Leu-zipper domain (aa 317-345). CEBP alpha  forms both homodimers, and heterodimers with other subfamily members. One alternative start site at Met120 generates a low activity 30-32 kDa isoform variant. There is also a utilized phosphorylation site at Ser21 that regulates activity. Over aa 1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Entrez Gene IDs

1050 (Human); 12606 (Mouse); 24252 (Rat)

Gene Symbol

CEBPA

UniProt

P49715 (Human)

Additional CEBP alpha Products

Product Documents for Human CEBP  alpha Antibody

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Product Specific Notices for Human CEBP  alpha Antibody

For research use only

Citations for Human CEBP  alpha Antibody

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Protocols

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