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Human CEBP  epsilon Antibody

Catalog #: MAB6726 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6726
MAB6726-SP
Detection of Human CEBP epsilon by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEBP  epsilon Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEBP  epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEBP alpha, beta, delta, gamma, or zeta is observed.
Source
Monoclonal Mouse IgG1 Clone # 695345
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CEBP  epsilon
Thr151-Arg210
Accession # Q15744
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CEBP e antibody by Western Blot. View Larger

Detection of Human CEBP epsilon by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human CEBP e Monoclonal Antibody (Catalog # MAB6726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP e at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Immunocytochemistry CEBP e antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

CEBP epsilon in HeLa Human Cell Line. CEBP e was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human CEBP e Monoclonal Antibody (Catalog # MAB6726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEBP epsilon

CCAAT-enhancer binding protein epsilon (CEBP epsilon ) is a 32 kDa basic-leucine zipper transcription factor that is expressed in myeloblasts, granulocytes, and eosinophils. Multiple isoforms of human CEBP epsilon are generated by alternate splicing and alternative translation initiation sites. All four isoforms contain the bZIP domain (aa 208-267) but exert different effects on myeloid differentiation. Full length CEBP epsilon and the 30 kDa isoform (aa 33-281) cooperate with c-Myb to activate the transcription of genes involved in myeloid differentiation. The 27 kDa isoform (which lacks aa 69-97) and the 14 kDa isoform (which begins at Met153) function as transcriptional repressors in eosinophils. Within aa 151-210, human CEBP epsilon shares 93% amino acid sequence identity with mouse and rat CEBP epsilon, respectively.

Long Name
CCAAT/enhancer-Binding Protein epsilon
Entrez Gene IDs
1053 (Human); 110794 (Mouse); 25410 (Rat)
Alternate Names
C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CEBP epsilon; CEBPE; CRP1C/EBP epsilon

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