Human CELSR3 PE-conjugated Antibody

Catalog #: FAB7278P Datasheet / COA / SDS

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FAB7278P

Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry.

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Human CELSR3 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CELSR3 in ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 763103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CELSR3
Val531-Ser711
Accession # Q9NYQ7

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human CELSR3 PE-conjugated Monoclonal Antibody (Catalog # FAB7278P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CELSR3

CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 98% aa sequence identity with mouse CELSR3.

Long Name

Cadherin, EGF LAG Seven-pass G-type Receptor 3

Entrez Gene IDs

1951 (Human); 107934 (Mouse); 83466 (Rat)

Alternate Names

Cadherin family member 11; cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila); CDHF11; CDHF11RESDA1; CELSR3; EGFL1; EGFL1cadherin EGF LAG seven-pass G-type receptor 3; EGF-like protein 1; EGF-like-domain, multiple 1; epidermal growth factor-like 1; Epidermal growth factor-like protein 1; Flamingo homolog 1; FMI1anchor protein; HFMI1; KIAA0812; MEGF2; MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog; multiple EGF-like domains 2; Multiple EGF-like domains protein 2; Multiple epidermal growth factor-like domains protein 2; RESDA1