Human Cerebellin-2 Antibody

Catalog #: MAB70441 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB70441
MAB70441-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cerebellin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cerebellin-2 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cerebellin-1 and rhCerebellin-4 is observed, and approximately 50% cross-reactivity with rhCerebellin-3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 704323

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-2
Arg50-Leu224
Accession # Q8IUK8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Cerebellin-2 in Human Brain. Cerebellin-2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB70441) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerebellin-2

Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), Human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin‑2.

Entrez Gene IDs

147381 (Human); 12405 (Mouse); 291388 (Rat)

Alternate Names

CBLN2; cerebellin 2 precursor; Cerebellin2; Cerebellin-2