Human Chemerin Biotinylated Antibody Summary
Glu21-Ser157
Accession # Q99969
Applications
Human Chemerin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chemerin
Human Chemerin, also known as Tazarotene-induced Gene 2, (TIG2) is a new, but distant member of the Cystatin superfamily (1‑3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin-fold containing domain, and a six aa C-terminal prosegment (1, 4). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the fold, and three potential sites for phosphorylation and one for myristoylation (5). The precursor molecule undergoes proteolytic processing at both termini by unknown proteases. The N-terminal residue 20 aa hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (1, 6). In either case, it gives rise to a soluble proform that undergoes further processing at the C-terminus. In human, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 21‑157) (7). A shorter 134 aa form has been described (5). Bioactivity seems to be concentrated in the nine residues preceding the prosegment (aa 149‑157). Retention of the prosegment blocks activity (4). The 137 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (7). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature human Chemerin shares 67% aa sequence identity with mouse Chemerin (7). There is apparently cross-species activity for the protein (8).
- Nagpal, S. et al., (1997) J. Invest. Dermatol. 109:91.
- Storici, P. et al., (1996) Eur. J. Biochem. 238:769.
- Zanetti, M., (2004) J. Leukoc. Biol. 75:39.
- Wittamer, V. et al., (2004) J. Biol. Chem. 279:9956.
- Meder, W. et al., (2003) FEBS Lett. 555:495.
- Yokoyama-Kobayashi, M. et al., (1999) Gene 228:161.
- Wittamer, V. et al., (2003) J. Exp. Med. 198:977.
- Busmann, A. et al., (2004) J. Chromatog. B 811:217.
Product Datasheets
Citations for Human Chemerin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Antimicrobial and Attractant Roles for Chemerin in the Oral Cavity during Inflammatory Gum Disease
Authors: U Godlewska, P Brzoza, A Sroka, P Majewski, H Jentsch, M Eckert, S Eick, J Potempa, BA Zabel, J Cichy
Front Immunol, 2017-03-29;8(0):353.
Species: Human
Sample Types: Serum
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A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Potential role of chemerin in recruitment of plasmacytoid dendritic cells to diseased skin.
Authors: Skrzeczynska-Moncznik J, Wawro K, Stefanska A, Oleszycka E, Kulig P, Zabel BA, Sulkowski M, Kapinska-Mrowiecka M, Czubak-Macugowska M, Butcher EC, Cichy J
Biochem. Biophys. Res. Commun., 2009-01-23;380(2):323-7.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Regulation of chemerin bioactivity by plasma carboxypeptidase N, carboxypeptidase B (activated thrombin-activable fibrinolysis inhibitor), and platelets.
Authors: Du XY, Zabel BA, Myles T, Allen SJ, Handel TM, Lee PP, Butcher EC, Leung LL
J. Biol. Chem., 2008-11-14;284(2):751-8.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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