Human Chemokine Luminex® Performance Assay 8-plex Fixed Panel
Discontinued Product
LKTM012 has been discontinued and is replaced by LKTM012B.
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Human Chemokine Luminex® Performance Assay 8-plex Fixed Panel Summary
| Assay Type | bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and antibody-coated beads |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. |
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Precision
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Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays. |
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| n=40 (20x2 levels) | n=25 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| CCL11/Eotaxin | 3.2% | 10.0% |
| CCL2/MCP-1 | 1.4% | 8.9% |
| CCL3/MIP-1alpha | 1.5% | 7.3% |
| CCL4/MIP-1beta | 3.0% | 9.6% |
| CXCL1/GRO alpha | 1.0% | 8.0% |
| CXCL10/IP-10 | 2.1% | 8.8% |
| CXCL8/IL-8 | 1.8% | 9.0% |
| RANTES | 4.7% | 17.4% |
Accuracy
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Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
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| Linearity | Spiked Recovery |
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|---|---|---|---|---|
| Analyte | N | Average % | N | Average % |
|
*Natural linearity |
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| CCL11/Eotaxin | 4 | 92% | 4 | 107% |
| CCL2/MCP-1 | 4* | 82% | High endogenous | |
| CCL3/MIP-1alpha | 4 | 77% | 4 | 139% |
| CCL4/MIP-1beta | 4 | 103% | 4 | 111% |
| CXCL1/GRO alpha | 4 | 80% | High endogenous | |
| CXCL10/IP-10 | 3 | 110% | High endogenous | |
| CXCL8/IL-8 | 4 | 118% | 4 | 96% |
| RANTES | 4* | 96% | High endogenous | |
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Scientific Data
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Human Chemokine Performance Luminex Panel Sample Values Serum samples from 20 apparently healthy individuals were run on the Human Chemokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.
Product Datasheets
Citation for Human Chemokine Luminex® Performance Assay 8-plex Fixed Panel
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Asymptomatic Malaria Infection Is Maintained by a Balanced Pro- and Anti-inflammatory Response
Authors: A Frimpong, J Amponsah, AS Adjokatseh, D Agyemang, L Bentum-Enn, EA Ofori, E Kyei-Baafo, K Akyea-Mens, B Adu, GI Mensah, LE Amoah, KA Kusi
Frontiers in Microbiology, 2020-11-17;11(0):559255.
Species: Human
Sample Types: Plasma
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