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Human ChemR23 PE-conjugated Antibody

Catalog #: FAB362P
1 Citation Datasheet / COA / SDS
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FAB362P
Detection of ChemR23 in Human Blood Monocytes by Flow Cytometry.
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Citations (1)
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Human ChemR23 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ChemR23. Stains human ChemR23-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG3 Clone # 84939
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human ChemR23
Met1-Leu371
Accession # NP_004063
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ChemR23 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of ChemR23 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PerCP-conjugated Monoclonal Antibody (FAB301C) and either (A) Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P) or (B) Mouse IgG3Phycoerythrin Isotype Control (IC007P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of ChemR23 in HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP by Flow Cytometry. HEK293 human cell line (A) transfected with human ChemR23 or (B) irrelevant protein and eGFP, were stained with Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P). Quadrant markers were set based on Mouse IgG3 PE Isotype Control (IC007P, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ChemR23

ChemR23 is a chemoattractant receptor expressed on dendritic cells and activated macrophages. ChemR23 binds Chemerin and functions as a coreceptor for SIV and some primary HIV-1 strains.

References
  1. Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.
Long Name
Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23
Entrez Gene IDs
1240 (Human); 14747 (Mouse); 60669 (Rat)
Alternate Names
CHEMERINR; chemokine receptor-like 1; chemokine-like receptor 1; ChemR23; cmklr1; Dez; Gpcr27; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; MGC126105; MGC126106; orphan G-protein coupled receptor, Dez

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Citation for Human ChemR23 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration
    Authors: Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A. Schneider, Medhanie A. Mulaw et al.
    Oncotarget

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