Human Chondrolectin Antibody

Name: Human Chondrolectin Antibody
Brand: R&D Systems
SKU: MAB2576
Rating: 5 (1 reviews)

Catalog #: MAB2576 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2576
MAB2576-SP

Detection of Chondrolectin in PC‑3 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chondrolectin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chondrolectin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 396305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chondrolectin
Arg22-Asn216
Accession # Q9H9P2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Chondrolectin

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Chondrolectin in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Immunocytochemistry

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Chondrolectin in PC‑3 Human Cell Line. Chondrolectin was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chondrolectin

Chondrolectin (CHODL) is a 36 kDa type I transmembrane protein containing a C-type lectin carbohydrate recognition domain. Multiple isoforms of human CHODL are generated as a result of alternative promoter usage and/or alternative splicing. Some isoforms are differentially expressed during T cell development. Human and mouse CHODL share 94% amino acid sequence identity in their extracellular region.

Entrez Gene IDs

140578 (Human); 246048 (Mouse)

Alternate Names

C21orf68; CHODL; Chondrolectin; chromosome 21 open reading frame 68; FLJ12627; MT75; PRED12; Transmembrane protein MT75