Human Chordin-like 2/CHRDL2 Antibody

Catalog #: MAB24481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24481
MAB24481-SP

Detection of Chordin‑like 2/CHRDL2 in MCF-7 cells by Flow Cytometry.

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Human Chordin-like 2/CHRDL2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CHRDL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 272905

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Chordin-like 2/CHRDL2

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

MCF‑7 human breast cancer cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Chordin‑like 2/CHRDL2 in MCF-7 cells by Flow Cytometry. MCF-7 cells were stained with Mouse Anti-Human Chordin‑like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB24481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Entrez Gene IDs

25884 (Human); 69121 (Mouse)

Alternate Names

BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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