Human Chromogranin B Antibody

Catalog #: MAB88681 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB88681-100
MAB88681-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chromogranin B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chromogranin B in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 914332R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Chromogranin B
Met1-Gly677
Accession # P05060

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Chromogranin B Monoclonal Antibody (Catalog # MAB88682).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin B

Chromogranin B (CHGB), also known as Secretogranin-1, is a 673 amino acid neuroendocrine secretory granule protein which is cleaved into three fragments: PE-11 (aa 575-585), GAWK peptide (aa 440-513) and CCB peptide (617-673). Human CHGB was originally isolated from human pheochromocytoma tissue, and is expressed in normal and malignant neuroendocrine tissues. CHGB peptides play a role in hormonal regulation in pancreatic islets, hypothalamus and pituitary gland. CHGB peptides, (aa 314-332 and 350-365) have been identified in several endocrine tumors including, gastrinoma, thyroid carcinoma, insulinoma, pancreatic endocrine tumor and pituitary adenomas.

Entrez Gene IDs

1114 (Human); 12653 (Mouse); 24259 (Rat)

Alternate Names

CgB; CHGB; chromogranin B (secretogranin 1); Chromogranin B; Chromogranin-B; SCG1; SCG1sgI; secretogranin B; Secretogranin I; secretogranin-1; SgI