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Human Chymase/CMA1 Antibody

Catalog #: MAB4099
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4099
MAB4099-SP
Detection of Human Chymase/CMA1 by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chymase/CMA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chymase/CMA1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) MCPT-1, rmMCPT-6, rmMCPT-7, or rmMCPT-11.
Source
Monoclonal Mouse IgG2A Clone # 422818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB40991).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Chymase/CMA1 DuoSet ELISA Kit (Catalog # DY4099-05) for convenient development of a sandwich ELISA.

 
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Chymase/CMA1 (Catalog # 4099-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Chymase/CMA1 antibody by Western Blot. View Larger

Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA View Larger

Human Chymase/CMA1 ELISA Standard Curve. Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chymase/CMA1

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References
  1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
  2. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
  3. Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
  4. Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.
Entrez Gene IDs
1215 (Human); 17228 (Mouse); 25627 (Rat)
Alternate Names
Alpha-chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Chymase; chymase, heart; chymase, mast cell; CMA1; CYH; CYM; EC 3.4.21; EC 3.4.21.39; Mast cell protease I; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891

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Citations for Human Chymase/CMA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload
    Authors: PC Powell, CC Wei, L Fu, B Pat, WE Bradley, JF Collawn, LJ Dell'Itali
    Heliyon, 2019-04-04;5(4):e01397.
    Species: Human, Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Chymase mediates angiotensin-(1-12) metabolism in normal human hearts
    Authors: Sarfaraz Ahmad, Chih-Chang Wei, Jose Tallaj, Louis J. Dell’Italia, Norihito Moniwa, Jasmina Varagic et al.
    Journal of the American Society of Hypertension
  3. Chymase-Dependent Generation of Angiotensin II from Angiotensin-(1-12) in Human Atrial Tissue
    Authors: Sarfaraz Ahmad, Tony Simmons, Jasmina Varagic, Norihito Moniwa, Mark C. Chappell, Carlos M. Ferrario
    PLoS ONE

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