Human CIN85/SH3KBP1 Antibody

Catalog #: MAB8877 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8877
MAB8877-SP

Detection of Human CIN85/SH3KBP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CIN85/SH3KBP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CIN85/SH3KBP1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 931308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CIN85/SH3KBP1
Pro366-Lys665
Accession # Q96B97

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CIN85/SH3KBP1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB8877) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIN85/SH3KBP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIN85/SH3KBP1

Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1). SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization. The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region. Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains. Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.

Long Name

Cbl-Interacting Protein of 85 KDa

Entrez Gene IDs

30011 (Human); 58194 (Mouse); 84357 (Rat)

Alternate Names

C-Cbl-Interacting Protein; CD2-binding protein 3; CD2BP3; CIN85; CIN85Cbl-interacting protein of 85 kDa; GIG10; HSB1; HSB-1; Human Src family kinase-binding protein 1; MIG18; migration-inducing gene 18; SH3 domain-containing kinase-binding protein 1; SH3-domain kinase binding protein 1; SH3KBP1; Src family kinase-binding protein 1; src-related kinase binding protein-1