Human CIN85/SH3KBP1 Antibody

Catalog #: MAB88771 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB88771
MAB88771-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CIN85/SH3KBP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CIN85/SH3KBP1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 931346

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CIN85/SH3KBP1
Pro366-Lys665
Accession # Q96B97

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CIN85/SH3KBP1 in A549 Human Cell Line. CIN85/SH3KBP1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB88771) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIN85/SH3KBP1

Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1). SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization. The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region. Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains. Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.

Long Name

Cbl-Interacting Protein of 85 KDa

Entrez Gene IDs

30011 (Human); 58194 (Mouse); 84357 (Rat)

Alternate Names

C-Cbl-Interacting Protein; CD2-binding protein 3; CD2BP3; CIN85; CIN85Cbl-interacting protein of 85 kDa; GIG10; HSB1; HSB-1; Human Src family kinase-binding protein 1; MIG18; migration-inducing gene 18; SH3 domain-containing kinase-binding protein 1; SH3-domain kinase binding protein 1; SH3KBP1; Src family kinase-binding protein 1; src-related kinase binding protein-1