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Human Claudin-1 Antibody

Catalog #: MAB4618
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4618
MAB4618-SP
Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-1 Antibody Summary

Species Reactivity
Human
Specificity
Stains human Claudin-1 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 421203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Claudin‑1 antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Entrez Gene IDs
9076 (Human); 12737 (Mouse); 65129 (Rat)
Alternate Names
claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1

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Citations for Human Claudin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry
    Authors: Peiqi Yin, Ye Li, Leiliang Zhang
    Journal of Virology
  2. Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy
    Authors: SP Hong, TE Chan, Y Lombardo, G Corleone, N Rotmensz, S Bravaccini, A Rocca, G Pruneri, KR McEwen, RC Coombes, I Barozzi, L Magnani
    Nat Commun, 2019-09-02;10(1):3840.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. BAFF and APRIL expression as an autoimmune signature of membranous nephropathy
    Authors: SS Han, SH Yang, HA Jo, YJ Oh, M Park, JY Kim, H Lee, JP Lee, SH Lee, KW Joo, CS Lim, YS Kim, DK Kim
    Oncotarget, 2017-12-14;9(3):3292-3302.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody.
    Authors: Mailly L, Xiao F, Lupberger J, Wilson G, Aubert P, Duong F, Calabrese D, Leboeuf C, Fofana I, Thumann C, Bandiera S, Lutgehetmann M, Volz T, Davis C, Harris H, Mee C, Girardi E, Chane-Woon-Ming B, Ericsson M, Fletcher N, Bartenschlager R, Pessaux P, Vercauteren K, Meuleman P, Villa P, Kaderali L, Pfeffer S, Heim M, Neunlist M, Zeisel M, Dandri M, McKeating J, Robinet E, Baumert T
    Nat Biotechnol, 2015-03-23;33(5):549-54.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  5. Hepatitis C virus induces CD81 and claudin-1 endocytosis.
    Authors: Farquhar MJ, Hu K, Harris HJ
    J. Virol., 2012-02-08;86(8):4305-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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