Human Claudin-15 Antibody

Catalog #: MAB10344 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10344-100
MAB10344-SP

Detection of Claudin-15 in HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP by Flow Cytometry.

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All Claudin-15 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Claudin-15 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 540315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-15
Accession # P56746

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Claudin-15 in HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Claudin-15 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Claudin-15 Monoclonal Antibody (Catalog # MAB10344) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-15

CLDN15 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.

Entrez Gene IDs

24146 (Human); 60363 (Mouse); 304388 (Rat)

Alternate Names

claudin 15; Claudin15; Claudin-15; CLDN15; FLJ42715; MGC19536