Human Claudin-17 Antibody

Catalog #: MAB4619
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4619
MAB4619-SP

Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry.

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All Claudin-17 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 410509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-17
Met1-Val224
Accession # P56750

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry

Detection of Claudin-17 antibody in A431 Human Cell Line antibody by Flow Cytometry.

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Detection of Claudin‑17 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-17

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.

Entrez Gene IDs

26285 (Human); 239931 (Mouse); 304125 (Rat)

Alternate Names

claudin 17; Claudin17; Claudin-17; CLDN17; human CLDN17 gene for claudin-17, 10claudin-17; MGC126552; MGC126554

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Claudin-17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis
Authors: S Izraely, S Ben-Menach, O Sagi-Assif, T Meshel, DM Marzese, S Ohe, I Zubrilov, M Pasmanik-C, DSB Hoon, IP Witz
Oncotarget, 2017-07-05;8(44):75778-75796.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry