Human CLEC17A Antibody

Catalog #: MAB101471 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB101471-100
MAB101471-SP

Detection of CLEC17A in HEK293 cells by Flow Cytometry.

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Human CLEC17A Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC17A in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1064719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO-derived human CLEC17A
Lys194-Cys378
Accession # Q6ZS10

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human CLEC17A and eGFP vs irrelevant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CLEC17A in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human CLEC17A and eGFP (A) vs irrelevant cells (B) were stained with eGFP and Mouse Anti-Human CLEC17A Monoclonal Antibody (Catalog # MAB101471) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2). It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca²⁺-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References