Home / CLEC2L / Human CLEC2L Antibody

Human CLEC2L Antibody

Catalog #: MAB10204 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10204-100
MAB10204-SP
Detection of CLEC2L in HEK293 Human Cell Line Transfected with Human CLEC2L and eGFP by Flow Cytometry
1 Image
Product Details
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC2L Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC2L in direct ELISA.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2326G
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human CLEC2L protein
Ser90-Thr214
Accession # P0C7M8-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.5 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CLEC2L in HEK293 Human Cell Line Transfected with Human CLEC2L and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP was stained with (A) Rabbit Anti-Human CLEC2L Monoclonal Antibody (Catalog # MAB2531) or (B) Rabbit IgG control antibody (MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References
  1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
  5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
  6. Lysenko, O. et al. (2013) PloS One. 8:e65345.
Long Name
C-type Lectin Domain Family 2, Member L
Entrez Gene IDs
154790 (Human); 665180 (Mouse); 296985 (Rat)
Alternate Names
CLEC2L; C-type lectin domain family 2 member L; C-type lectin domain family 2, member L

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CLEC2L Antibody

There are currently no reviews for this product. Be the first to review Human CLEC2L Antibody and earn rewards!

Have you used Human CLEC2L Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review