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Human CLEC4D/CLECSF8 PE-conjugated Antibody

Catalog #: FAB2806P
3 Citations Datasheet / COA / SDS
Clone 413512 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2806P
Detection of CLEC4D/CLECSF8 in Human Blood Monocytes by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC4D/CLECSF8 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC4D/CLECSF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF9, rhCLECSF13, rhOCIL, or rhOCILrp2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 413512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC4D/CLECSF8
Gly52-Asn215
Accession # Q8WXI8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLEC4D/CLECSF8 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CLEC4D/CLECSF8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CLEC4D/CLECSF8 PE-conjugated Monoclonal Antibody (Catalog # FAB2806P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC4D/CLECSF8

CLEC4D (C-type lectin domain family 4 member D), also known as CLECSF8, CLEC-6, and MCL, is a 30 kDa type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215 amino acid (aa) protein that contains a 17 aa N-terminal cytoplasmic domain, a 21 aa TM segment, and a 177 aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118 aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Long Name
C-type Lectin Domain Family 4, Member D
Entrez Gene IDs
338339 (Human); 17474 (Mouse)
Alternate Names
CD368; CLEC4D; CLEC6; CLEC-6; CLECSF8; CLECSF8Mpcl; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 8; C-type lectin domain family 4 member D; C-type lectin domain family 4, member D; C-type lectin receptor; C-type lectin superfamily member 8; C-type lectin-like receptor 6; macrophage C-type lectin; MCL; MGC40078; Mpcl

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Citations for Human CLEC4D/CLECSF8 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Identification of HIV transmitting CD11c+ human epidermal dendritic cells
    Authors: KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman
    Nat Commun, 2019-06-21;10(1):2759.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
    Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
    J. Leukoc. Biol, 2017-03-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. New insights into the phenotype of human dendritic cell populations.
    Authors: Clark GJ et al.
    Clin Transl Immunology

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