Human CLEC4E Antibody

Catalog #: MAB8995 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8995-100
MAB8995-SP

Detection of CLEC4e in HEK293 Human Cell Line Transfected with Human CLEC4e and eGFP by Flow Cytometry.

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All CLEC4E Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC4E Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC4E in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2455C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived recombinant human CLEC4E
Arg41-Leu219
Accession # Q9ULY5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CLEC4e in HEK293 Human Cell Line Transfected with Human CLEC4e and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CLEC4e or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human CLEC4e Monoclonal Antibody (Catalog # MAB8995) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4E

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

References

Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
Wells, C.A. et al. (2008) J. Immunol. 180:7404.
Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
Ostrop, J. et al. (2015) J. Immunol. 195:2417.
Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Long Name

C-type Lectin Domain Family 4, Member E

Entrez Gene IDs

26253 (Human); 56619 (Mouse); 450223 (Rat)

Alternate Names

CLEC4E; CLECSF9; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9; C-type lectin domain family 4 member E; C-type lectin domain family 4, member E; Macrophage-inducible C-type lectin; MINCLE; MINCLEC-type lectin superfamily member 9