Human Coagulation Factor III/
Tissue Factor Antibody
Human Coagulation Factor III/
Tissue Factor Antibody Summary
Gly34-Glu251
Accession # P13726
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Coagulation Factor III/Tissue Factor by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Coagulation Factor III/Tissue Factor at approximately 48 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).
- Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
- Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
- Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
- Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
- Morrissey, J.H. et al. (1987) Cell 50:129.
- Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Product Datasheets
Citations for Human Coagulation Factor III/
Tissue Factor Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
Authors: Kinchen J, Chen HH, Parikh K et al.
Cell
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The Proteome of Extracellular Vesicles Released from Pulmonary Microvascular Endothelium Reveals Impact of Oxygen Conditions on Biotrauma
Authors: Schaubmayr, W;Hochreiter, B;Hunyadi-Gulyas, E;Riegler, L;Schmidt, K;Tiboldi, A;Moser, B;Klein, KU;Krenn, K;Scharbert, G;Mohr, T;Schmid, JA;Spittler, A;Tretter, V;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Electron Microscopy -
Evaluation of four commercial ELISAs to measure tissue factor in human plasma
Authors: Ana T.A. Sachetto, Sierra J. Archibald, Ravi Bhatia, Dougald Monroe, Yohei Hisada, Nigel Mackman
Research and Practice in Thrombosis and Haemostasis
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Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C
Authors: C Mwase, TN Phung, MJ O'Sullivan, JA Mitchel, M De Marzio, A K?l?ç, ST Weiss, JJ Fredberg, JA Park
Cells, 2022-01-13;11(2):.
Species: Human
Sample Types: Extracellular Vesicles
Applications: Western Blot -
PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose Availability
Authors: TLR Humphries, K Shen, A Iyer, DW Johnson, GC Gobe, D Nikolic-Pa, DP Fairlie, DA Vesey
International Journal of Molecular Sciences, 2021-07-14;22(14):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Human Vascular Injury‐on‐a‐Chip Model of Hemostasis
Authors: Izmarie Poventud‐Fuentes, Keon Woo Kwon, Jeongyun Seo, Maurizio Tomaiuolo, Timothy J. Stalker, Lawrence F. Brass et al.
Small
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PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood Clotting
Authors: A Iyer, TLR Humphries, EP Owens, KN Zhao, PP Masci, DW Johnson, D Nikolic-Pa, GC Gobe, DP Fairlie, DA Vesey
Frontiers in Physiology, 2021-03-10;12(0):615428.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent manner
Authors: Desirée Edén, Grigorios Panagiotou, Dariush Mokhtari, Jan W. Eriksson, Mikael Åberg, Agneta Siegbahn
Upsala Journal of Medical Sciences
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The Low Molecular Weight Heparin Tinzaparin Attenuates Platelet Activation in Terms of Metastatic Niche Formation by Coagulation-Dependent and Independent Pathways
Authors: LM Gockel, JM Ponert, S Schwarz, M Schlesinge, G Bendas
Molecules, 2018-10-24;23(11):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sensitive and Specific Detection of Platelet-Derived and Tissue Factor–Positive Extracellular Vesicles in Plasma Using Solid-Phase Proximity Ligation Assay
Authors: Åsa Thulin, Junhong Yan, Mikael Åberg, Christina Christersson, Masood Kamali-Moghaddam, Agneta Siegbahn
TH Open
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Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
Sci Rep, 2016-05-04;6(0):25171.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Fixed single-cell transcriptomic characterization of human radial glial diversity
Authors: Elliot R. Thomsen, John K. Mich, Zizhen Yao, Rebecca D. Hodge, Adele M. Doyle, Sumin Jang et al.
Nature Methods
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Interferon-gamma and tumor necrosis factor-alpha act synergistically to up-regulate tissue factor in alveolar epithelial cells
Authors: Julie A. Bastarache, Sara C. Sebag, Brandon S. Grove, Lorraine B. Ware
Experimental Lung Research
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Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011-12-12;6(12):e28540.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
Authors: Kelly OG, Chan MY, Martinson LA
Nat. Biotechnol., 2011-07-31;29(8):750-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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Tissue Factor Antibody
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Used as detection antibody after labeling with Sulfo-Tag according to manufacturer's protocol (Meso Scale Diagnostics LLC). Detection range – 1.5-25,000 pg/ml
